Budi Baktijasa Dharmadjati, Djanggan Sargowo, Aulanniam Aulanniam, Budi Susetyo Pikir, Oryza Sativa, Kandita Arjani, Muhammad Rifqi Djamal Hasan
Introduction: Adult-adipose tissue produce mesenchymal stem cells (MSCs), known as adipose-derived mesenchymal stem cells (AMSCs). Unlike other source of stem cell (SC), adipose tissue has abundant availability and easy to obtained without any conflicting ethical issues. The aim of the study is to demonstrate that each part of adipose tissue will generates SC with similar MSCs characteristic and specific markers thus no part of it will be waste. Methods: Using one-way experimental design, 20 grams of subcutaneous adipose tissue were cultured with different methods to obtained 25 samples that divided into five different group. Sterile gauze, a 100 m and 70 m strainer were used in filtration step of culture process. Cell morphology were observed by inverted microscope. Fluorescence microscope was used to analyze expression of CD34, CD45, CD90, and CD105. Results: At passage 1, every group adhered to plastic surface with fibroblastic-like morphology in 3rd day. CD34 and CD45 expressions were detected in control group, and gradually decreased among another groups whereas lack of CD45 expression were consistent on other remaining groups. The expression of CD90 and CD105 were visibly increased significantly from control group to fourth group. Conclusion: This study demonstrated that either SVF and/or supernatant generates fibroblastic-like cells and surface markers expression that similar to MSCs criteria. Although supernatant still produce better SC than SVF. Therefore, every parts of adipose tissue are an appealing alternative of stem cell source from any available culture methods. © 2022 UPM Press. All rights reserved.
Department of Cardiology and Vascular Medicine, Faculty of Medicine, Airlangga University, Surabaya, 60131, Indonesia; Department of Cardiology and Vascular Medicine, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia; Biochemistry Laboratory, Department of Chemistry, Faculty of Sciences, Brawijaya University, Malang, 65145, Indonesia