Andi Rezda Sri Mulyani, Trinil Susilawati, Achadiah Rachmawati, Irida Novianti, Anny Amalia, Deny Sulistyowati, Hikma Nur Azizah, Tsania Syifatuzzahra, Mashitah Shikh, Maidin Maidin, Aulia Puspita Anugra Yekti
This study aims to evaluate the effect of different gradients on the quality and proportion of sexed semen using the egg white albumin sedimentation method. This study was conducted at the Singosari Artificial Insemination Center in Malang, using Friesian Holstein semen with an average fresh motility of >70%. The study was conducted experimentally with 10 replicates and 2 treatments, namely T1 = egg white sedimentation sexing with 2 gradients (30% and 50%) and T2 = egg white sedimentation sexing with 3 gradients (30%, 40%, and 50%). The parameters observed included motility, viability, abnormality, membrane integrity, and the proportion of X and Y chromosomes. The quality evaluation results were analyzed using an independent t-test, while the determination of the spermatozoa proportion was analyzed using the Chi-Square test. The results showed that differences in gradients did not have a significant effect (p < 0.05) on any quality parameters in each layer. In addition, the proportion of X and Y spermatozoa also showed no difference between the upper and lower fractions in both layers (p > 0.05). In conclusion, spermatozoa separation using 3-gradient egg white albumin sedimentation tended to provide more efficient separation while maintaining spermatozoa quality, although no significant differences were observed compared to the 2-gradient method. © The Authors, published by EDP Sciences, 2026.
Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, Indonesia; Undergraduate Student, Department of Animal Science, Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, Indonesia; Singosari National Artificial Insemination Center, Malang, 65153, Indonesia; Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, Indonesia; Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Selangor, Serdang, Malaysia; Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia, 43400 UPM, Selangor, Serdang, Malaysia
