Djalal Rosyidi, Lilik E. Radiati, Dahliatul Qosimah, Indah A. Amri, Dodik Prasetyo, Agri K. Anisa
Coffee (Coffea canephora) is one of the world's most popular beverages containing active chemicals such as caffeine, phenol, trigonelline, diterpenes, and water-soluble substances. Chlorogenic acids are a type of phenol found in coffee that can stimulate the immune system within the body. As a result, this natural substance promotes poultry growth by strengthening the immune system and preventing disease. There is a dearth of information on coffee as an immunomodulator in chickens, necessitating this investigation. The present study was therefore conducted to investigate the effect of Lampung Robusta green coffee extract on T lymphocyte cell activation in ISA-brown laying chickens. Ethanolic extract of the Lampung Robusta green coffee was prepared and subjected to liquid chromatography-mass spectrometric analysis. One-day-old ISA-brown laying chickens were divided into four groups: control (C), T1, T2, and T3 groups, which received extract coffee doses of 500, 1000, and 1500 mg/kg BW, respectively. The relative numbers of TCD4+, CD8+, and CD45+ cells were determined using a flow cytometer. The results of the LC-MS analysis revealed that the Lampung Robusta green coffee extract also contained chlorogenic acids. With a dose of 1500 mg/kg BW, Lampung Robusta green coffee extract had an immunomodulatory effect on the T3 group, increasing the relative number of TCD4+ and CD45+ while decreasing CD8+. The relative number of TCD8+ was higher than that of TCD4+, suggesting immunosuppressive potential. The findings of this study revealed that Lampung Robusta green coffee extract is effective as an immunomodulator in chickens. © 2022 Rosyidi et at.
Animal Product Technology Laboratory, Faculty of Animal Science, Universitas Brawijaya, Malang, Indonesia; Laboratory of Microbiology and Immunology, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia; Animal Clinical, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia; Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia
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