Water quality management for in-vitro test of recombinant vaccines on cantang grouper (Epinephelus sp.)

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Uun Yanuhar, Diana Arfiati, Muhammad Musa, Choirul Huda, Nur Sakinah Junirahma, Nico Rahman Caesar

2022 AIP Conference Proceedings Vol. 2513 Conference paper Cited by 0 SDG 6SDG 17 Quartile

Abstract

There is a close relationship between water quality conditions in the maintenance system and the health of cantang grouper (Epinephelus sp.). The main triggers of water quality are the following suboptimal levels were temperature, salinity, pH, and dissolved oxygen. Therefore, it is necessary to carry out water quality management in grouper rearing pond that is vaccinated to create controlled environmental conditions. The method used in this research is experimental. Water quality monitoring was carried out in all treatment tanks used for recombinant vaccines test, there were (A) Normal fish without treatment; (B) VNN-Infected fish; (C) Healthy fish with 33 μl vaccine treatment; (D) Healthy fish with 66 μl vaccine treatment; (E) Healthy fish with 112 μl vaccine treatment; (F) VNN-Infected fish with 33 μl vaccine treatment; (G) VNN-Infected fish with 66 μl vaccine treatment; and (H) VNN-Infected fish with 112 μl vaccine treatment; (I) Healthy fish with 33 μl vaccine treatment then infected with VNN; (J) Healthy fish treated with 66 μl vaccine then infected with VNN; (K) Healthy fish with 112 μl vaccine treatment then infected with VNN. Water quality control was carried out during the recombinant vaccine treatment process by adding an aerator for oxygen supply and heater to keep the temperature stable. The results of water quality temperature parameters were obtained in the range of 30-31°C, the salinity of 30 ppt, pH 7.5-7.8 and dissolved oxygen between 17.2-19.1 mg L-1. The water quality condition in grouper tanks with vaccine treatments was good and following the water quality standards that supports the life of cantang grouper. Water temperature has a major influence on the initiation and transmission of several fish diseases. The water quality in this study showed optimal values, thus supporting controlled water quality conditions during the recombinant vaccine treatment. © 2022 Author(s).

Affiliations

Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, University of Brawijaya, Malang, Jl. Veteran, Malang, East Java, 65145, Indonesia; Master Program of Aquaculture, Faculty of Fisheries and Marine Science, University of Brawijaya, Jl. Veteran, Malang, 65145, Indonesia; Doctoral Program of Environmental Science, Postgraduate Program, University of Brawijaya, Jl. Veteran, Malang, 65145, Indonesia

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