Mm. Riyaniarti Estri Wuryandari, Ninis Yuliati, Ekawati Sutikno, Hartati Tuna, Nita Damayanti, Muhaimin Rifa’i, Mochammad Fitri Atho’illah, Rizky Dzariyani Laili
Interleukin-21(IL-21) and interleukin-22(IL-22) expressions in the substantial intestine increase when inflammation occurs. They can also induce the production of pro-inflammatory cytokines and Matrix Metallo Proteinases (MMP) in subepithelial fibroblasts. To evaluate at IL-21 and IL-22 expressions in BALB/c mice after administering red M. oleifera leaf fermented extracts exposed to Salmonella typhi. The expression of IL-21 and IL-22 were evaluated for immunomodulatory effect was analyzed by flowcytometer. Data were analyzed by one-way ANOVA and continued with the Tukey test (p<0.05). The results showed that the fermented extract of red M. oleifera leaves could act as an immunosuppressor characterized by decreased levels of IL-21 and IL-22 both on CD4 T and CD8 T cells in mice injected with Salmonella typhi ranging from 14 mg/Kg BW to 42 mg/Kg BW and Pyrex analysis explains that the cathecin compound has the same active side as 8MR in forming bonds with MMP-9. The results of this study provide is the fermented extract of red M. oleifera leaves decreasing the expression of CD4+IL21+, CD8+IL21+ and CD4+IL22+, CD8+IL22+ through inhibition of MMP-9. © RJPT All right reserved.
Department of Pharmacy, Faculty of Pharmacy, Institut Ilmu Kesehatan Bhakti Wiyata, East Java, Kediri, 64114, Indonesia; Department of D3 Medical Laboratory Technology, Faculty of Tecnology and Health Management, Institut Ilmu Kesehatan Bhakti Wiyata, East Java, Kediri, 64114, Indonesia; Department of D4 Medical Laboratory Technology, Faculty of Tecnology and Health Management, Institut Ilmu Kesehatan Bhakti Wiyata, East Java, Kediri, 64114, Indonesia; Departement of Dentistry, Faculty Dental, Institut Ilmu Kesehatan Bhakti Wiyata, East Java, Kediri, 64114, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, East Java, Malang, 65145, Indonesia; Department of Nutrition, Sekolah Tinggi Ilmu Kesehatan Hang Tuah Surabaya, East Java, Surabaya, 60244, Indonesia