Ertika Fitri Lisnanti, Widya Paramita Lokapirnasari, Mohammad Anam Al Arif, Eka Pramyrtha Hestianah, Aswin Rafif Khairullah, Iwan Sahrial Hamid, Wiwiek Tyasningsih, Mirni Lamid, Mudhita Zikkrullah Ritonga, Farida Subasnia Dwijayanti, Amiril Mukmin, Saifur Rehman, Viski Fitri Hendrawan, Miarsono Sigit
Background and Aim: Avian pathogenic Escherichia coli (APEC) is a major cause of colibacillosis in broiler chickens, leading to systemic inflammation, organ dysfunction, disrupted lipid metabolism, and compromised meat quality. Growing concerns about antimicrobial resistance necessitate the development of sustainable alternatives to antibiotic growth promoters. Myrmecodia sp., a medicinal plant rich in bioactive flavonoids, tannins, and phenolics, has demonstrated antibacterial and antioxidant properties. However, limited information is available on the comparative effects of different preparation forms on organ function and lipid metabolism under APEC challenge conditions. This study aimed to evaluate the effects of Myrmecodia sp. extract and infusion on liver and kidney biomarkers, serum lipid profile, and meat lipid composition in APEC-infected broiler chickens. Materials and Methods: A total of 56 male Lohmann MB 202 broiler chickens were randomly assigned to seven groups: negative control, positive APEC-infected control, antibiotic control (zinc bacitracin), two extract treatments (15% and 30%), and two infusion treatments (1% and 2%). Treatments were administered from day 8 to day 35, and APEC infection was induced orally on day 21. Measured parameters included serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), blood urea nitrogen (BUN), creatinine, low-density lipoprotein (LDL), high-density lipoprotein (HDL), and meat LDL and HDL. Data were analyzed using one-way analysis of variance followed by Duncan’s multiple range test (p < 0.05). Results: APEC infection significantly increased SGPT, SGOT, BUN, creatinine, and LDL levels while decreasing HDL levels (p < 0.05). Supplementation with Myrmecodia sp. extract and infusion significantly improved all evaluated parameters compared with the positive control (p < 0.05). Among treatments, the 1% infusion consistently produced the most favorable effects, including reduced liver enzyme activity, improved renal function, decreased LDL levels, and increased HDL levels in both serum and meat. Conclusion: Myrmecodia sp., particularly in infusion form, demonstrated protective effects on organ function and lipid metabolism in broilers infected with APEC. These findings support its potential as a phytogenic alternative to antibiotics in sustainable poultry production systems within a One Health framework. © Lisnanti, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/)
Doctoral Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, East Java,, Surabaya, 60115, Indonesia; Faculty of Agriculture, Universitas Islam Kadiri, Kediri. Jl. Sersan Suharmaji 38, East Java, Kediri, 64128, Indonesia; Department of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Mulyorejo, East Java, Surabaya, 60115, Indonesia; Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Mulyorejo, East Java, Surabaya, 60115, Indonesia; Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Jl. Raya Bogor Km. 46 Cibinong, West Java, Bogor, 16911, Indonesia; Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Mulyorejo, East Java, Surabaya, 60115, Indonesia; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Mulyorejo, East Java, Surabaya, 60115, Indonesia; Laboratory of Anatomy, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Jl. Teuku Nyak Arief No. 441, Kopelma Darussalam, Aceh, Banda Aceh, 23111, Indonesia; Department of Pathobiology, Faculty of Veterinary and Animal Sciences, Gomal University, RV9W+GVJ, Indus HWY, Dera Ismail Khan, 27000, Pakistan; Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Brawijaya, Jl. Puncak Dieng, Kunci, Kalisongo, Dau, East Java, Malang, 65151, Indonesia; Faculty of Veterinary Medicine, Universitas Wijaya Kusuma Surabaya, Jl. Dukuh Kupang XXV No. 54, Dukuh Kupang, Dukuh Pakis, East Java, Surabaya, 60225, Indonesia
