Muhammad Sarmad, Gong Fayuan, Ren Tao, Eko Widodo, Rizki Prafitri, Osfar Sjofjan
Hematological parameters were evaluated in a feeding trial using one-day-old Hy-Line Brown strain layer chicks supplemented with GroPro, a yeast-based natural feed additive, under open house conditions. A total of 480 chicks were randomly allocated to four dietary treatments with six replicates per treatment (20 birds/replicate) using a Completely Randomized Design. The treatments consisted of a basal diet (T0), and diets supplemented feed additive with 0.2% (T1), 0.5% (T2), and 1.0% (T3) GroPro during seven weeks. At the end of the trial, blood samples were collected from the brachial vein and analyzed for hematological indices. Data were analyzed using ANOVA followed by Duncan's Multiple Range Test when significant differences were detected. The results showed that dietary GroPro significantly (P<0.01) influenced hemoglobin (Hb), red blood cell (RBC), white blood cell (WBC), packed cell volume (PCV), mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH). No significant effect (P>0.05) was observed on mean corpuscular hemoglobin concentration (MCHC). The inclusion of 1.0% GroPro resulted in the most favorable hematological profile, indicating improved oxygen-carrying capacity and immune status in starter-phase laying hens. © The Authors, published by EDP Sciences, 2026.
Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, Indonesia; Angel Yeast Co. Ltd., 168 Chengdong Avenue, Hubei, Yichang, 443003, China
