Kunti Tirtasari, Lucia Tri Suwanti, Hani Plumeriastuti, Rochmah Kurnijasanti, Mufasirin Mufasirin, Eka Pramyrtha Hestianah, Erma Safitri, Poedji Hastutiek, Alfiana Laili Dwi Agustin, Venri Novryantoro, Anissa Septiara, Suryo Kuncorojakti, Heni Puspitasari, Elly Nur Indasari, Lita Rakhma Yustinasari
| Toxoplasma gondii can cause an increase the cytokine, increased cytokine pro-inflamation can cause cell damage. Natural products such as propolis can be choice for treatment toxoplasmosis for reduce cytokine pro-inflamation (TNF-α and IFN-γ). We compare the amount of cytokines in each treatment group. We employed flow cytometry to assess cytokine expression. We used 36 mice were divided into six group, group one mice were not infected and treated as negative control, groups 2 to 6 allmice were infected T. gondii with 100 tachyzoites. Group 2 mice were infected but not treated, group 3 mice were infected and treated with orally cotrimoxazole 40 mg/kg body weight, group 4 mice were infected and treated with Ethanol Extract Propolis (EEP) 1 mg/mouse, group 5 mice were infected and treated with EEP 2 mg/mouse, and group 6 mice were infected and treated with EEP 4 mg/mouse one day after infection for three days. We observed cytokines in mice exposed to Toxoplasma gondii. Statistical analysis was performed with one-way ANOVA and Duncan Multiple Range Test in SPSS 30 software. Results showed that EEP (1–4 mg) can reduce reduced levels of pro-inflammatory cytokines (TNF-α, IFN-γ) no significant differences caspase-3 in all treatments, TNF-α and IFN-γ from the results we obtained showed lower values when compared to the group of toxoplasmosis mice treated using cotrimoxazole. The best choice for toxoplasmosis therapy using EEP is in group P6 with a dose of 4 mg. 2026 by the authors.
Doctoral Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia; Faculty of Veterinary Medicine, Universitas Pendidikan Mandalika, Indonesia; Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia; Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia; Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia; Veterinary Internal Medicine Laboratory, Faculty of Veterinary Medicine, Universitas Brawijaya, Indonesia
