A.N. Vidyaningrum, A.A. Arif, W.A. Septian, R.F. Putri, A. Ardiantoro, C.D. Nugraha, A.K. Thiruvenkadan, Suyadi Suyadi
The use of antioxidants in semen extenders plays a crucial role in preventing quality deterioration during dilution and storage. This study investigates the effects of quercetin supplementation in Tris-Aminomethane egg yolk extender on the semen quality of Dorper and Awassi rams stored at room temperature. Semen was collected twice weekly from Dorper and Awassi rams using an artificial vagina. A factorial experimental design was applied to evaluate the impact of quercetin concentrations (0, 30, 60, and 90 µM) and storage durations (0, 2, 4, 6, and 8 hours) at room temperature.The assessed parameters included individual motility, viability, abnormality, and membrane integrity. Data were analyzed using ANOVA and Duncan’s multiple range test. The results indicated that quercetin significantly enhanced individual motility in Dorper (P < 0.01) and Awassi (P < 0.05) rams, with the optimal effect observed at 30 µM. However, viability, abnormality, and membrane integrity were not significantly affected by quercetin supplementation. Storage duration significantly influenced motility and membrane integrity in both breeds (P < 0.01). The addition of 30 µM quercetin maintained sperm motility for up to 8 hours at room temperature, with motility rates of 76.4 ± 5.11% (Dorper) and 74.6 ± 8.28% (Awassi). In conclusion, quercetin supplementation at 30 µM in Tris-Aminomethane extender effectively preserves the semen quality of Dorper and Awassi rams during room temperature storage, with potential applications for improving artificial insemination success rates. 2026 by the authors.
Departement of Animal Reproduction and Genetic, Faculty of Animal Science, Universitas Brawijaya, Jl. Veteran, Malang, 65145, Indonesia; Departement of Animal Production, Faculty of Animal Science, Universitas Brawijaya, Jl. Veteran, Malang, 65145, Indonesia; Biotechnology Laboratory, Faculty of Animal Science, Universitas Brawijaya, Jl. Veteran, Malang, 65145, Indonesia; National Agency for Research and Innovation (BRIN), of The Republic of Indonesia, Indonesia; Department of Animal Genetics and Breeding, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu, Theni, 625602, India
