A'liyatur Rosyidah, Debora C. Purbani, Riyona D. Pratiwi, Sjaikhurrizal E. Muttaqien, Nawarat Nantapong, Mega F. Warsito, Muhammad N. Fikri, Feliadewi Ruth, Nunik Gustini, Gita Syahputra, Mohamad Padri, Diah R. Noerdjito, Arif Nurkanto, Hikmah Afani
Gold nanoparticles (AuNPs) synthesized through green synthesis have the potential for application in various biomedical sectors. This study demonstrates the optimization, synthesis, and characterization of AuNPs using marine microalga, Synechococcus moorigangae strain InaCC M208, isolated from Jakarta Bay, Indonesia. Optimization of the synthesis process focused on three parameters: temperature, ratio of precursor-to-extract, and incubation time. The AuNPs were characterized using various techniques, including UV–Vis spectroscopy, TEM, XRD, FT-IR, PSA, and EDX spectroscopy. The ethanol extract of S. moorigangae InaCC M208 was responsible for the reducing and stabilizing agent during the AuNPs synthesis. The optimized conditions for synthesis were found to be a precursor-to-extract ratio of 1:1 at 37 °C for 22 h. The UV–Vis spectra of the synthesized AuNPs (Syn-AuNPs) exhibited a surface plasmon resonance at 552 nm. According to FT-IR, various phytoconstituents from the extract contributed to the reduction and stabilization of Syn-AuNPs. Syn-AuNPs exhibited a spherical form with an average size of 8.2 nm and a zeta potential of −21.5 mV. Notably, these Syn-AuNPs exhibited antibacterial efficacy against the pathogenic Staphylococcus aureus InaCCB4, Escherichia coli InaCC B5, Klebsiella pneumoniae 1617, Pseudomonas aeruginosa N90PS, and E. coli 8654. Additionally, Syn-AuNPs also demonstrated antioxidant capacities against DPPH radicals. It is possible to readily scale up this environmentally friendly synthesis technique for future studies, and AuNPs produced through microalgae-mediated green synthesis have substantial promise for nanomedicine applications. © 2024 The Authors
Research Center for Vaccine and Drug, National Research and Innovation Agency (BRIN), West Java, Bogor, Indonesia; Research Center for Biosystematic and Evolution, National Research and Innovation Agency (BRIN), West Java, Bogor, Indonesia; School of Preclinical Science, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand; Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), West Java, Bogor, Indonesia; Department of Biology, Faculty of Mathematics and Natural Science, Brawijaya University, East Java, Malang, Indonesia; Department of Pharmaceutical Science, Faculty of Pharmacy, Airlangga University, East Java, Surabaya, Indonesia; Development of Algal Biotechnology in Kingdom of Saudi Arabia (DAB-KSA) Project, Beacon Development, King Abdullah University of Science and Technology (KAUST), Thuwal, Mecca, Saudi Arabia; Department of Biology, Faculty of Mathematics and Natural Science, University of Indonesia, West Java, Depok, Indonesia
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