Alexander Patera Nugraha, Ida Bagus Narmada, Ervina Restiwulan Winoto, I Gusti Aju Wahju Ardani, Ari Triwardhani, Alida Alida, Adya Pramusita, Reyhan Mahendra Nur, Nuraini Indrastie, Hui Yin Nam, Igo Syaiful Ihsan, Wibi Riawan, Fedik Abdul Rantam, Albertus Putera Nugraha, Tengku Natasha Eleena Binti Tengku Ahmad Noor
Objectives The aim of this article was to investigate Osterix, ALP, and osteopontin expression in the compression and tension sides of alveolar bone after the application of normoxic/hypoxic-preconditioned GMSCs in rabbits (Oryctolagus cuniculus) induced with OMF. Materials and Methods Forty-eight healthy, young male rabbits were divided into four groups: [-] OMF; [+] OMF; OMF with GMSCs normoxic-preconditioned; and OMF and GMSCs hypoxic-preconditioned. The central incisor and left mandibular molar in the experimental animals were moved, the mandibular first molar was moved mesially using nickel titanium (NiTi) and stainless steel ligature wire connected to a 50 g/mm 2 light force closed coil spring. Allogeneic application of normoxic or hypoxic-preconditioned GMSCs was used in as many as 10 6 cells in a 20 μL phosphate buffered saline single dose and injected into experimental animals' gingiva after 1 day of OTM. On days 7, 14, and 28, all experimental animals were euthanized. Osterix, ALP, and osteopontin expressions were examined by immunohistochemistry. Results Osterix, ALP, and osteopontin expressions were significantly different after allogeneic application of hypoxic-preconditioned GMSCs than normoxic-preconditioned GMSCs in the tension and compression of the alveolar bone side during OMF (p < 0.05). Conclusion Osterix, ALP, and osteopontin expressions were significantly more enhanced post-transplantation of GMSCs with hypoxic-preconditioning than after transplantation of normoxic-preconditioned GMSCs in rabbits (O. cuniculus) induced with OMF. © 2023. The Author(s).
Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; Nanotechnology and Catalysis Research Centre (NANOCAT), Universiti Malaya, Kuala Lumpur, Malaysia; Tissue Engineering Group, Department of Orthopaedic Surgery (NOCERAL), Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia; Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, Indonesia; Biomolecular Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia; Laboratory of Immunology and Virology, Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia; Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Membership of Faculty of Dental Surgery, Royal Collage of Surgeon, Edinburgh University, United Kingdom; Malaysian Armed Forces Dental Officer, 609 Armed Forces Dental Clinic, Kem Semenggo, Kuching, Sarawak, Malaysia