Hartati Hartati, Widya Pintaka Bayu Putra, Mariyono Mariyono, Muhammad Luthfi, Lukman Affandhy, Dewi Khosiya Robba, Eko Handiwirawan, Sulistiyoningtiyas Irmawanti, Aryogi Aryogi, Susan Maphilindawati Noor, Endang Romjali, Wike Andre Septian
Objectives: This study aimed to identify a 21 bp insertion/deletion mutation in the GSN gene (intron 2) and a single nucleotide polymorphism (c.48333G > A) in the PLAG1 gene (exon 3), and evaluate their association with growth traits in mature Madura and Bali cattle. Materials and Methods: A total of 219 animals (156 Madura and 73 Bali cattle) were assessed for body weight (BW) and four body measurements: Heart girth (HG), body length (BL), hip height (HH), and withers height (WH). Genotyping was performed using PCR and PCR-RFLP techniques. Results: Both genes were polymorphic, with high PIC values (> 0.30). The GSN gene polymorphism was significantly associated with HH in Bali cattle, while the PLAG1 gene showed significant associations with BW in both breeds. Conclusions: These findings suggest that PLAG1 (c.48333G > A) is a promising genetic marker for BW in Indonesian tropical cattle. © The authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0)
Research Center for Animal Husbandry, National Research and Innovation Agency (BRIN), Cibinong, Bogor, Indonesia; Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Cibinong, Bogor, Indonesia; Hydrodynamics Laboratory, National Research and Innovation Agency (BRIN), Cibinong, Bogor, Indonesia; Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Cibinong, Bogor, Indonesia; Animal Biotechnology, Faculty of Animal Science, Brawijaya University, Malang, Indonesia
