Dwi Gusmalawati, Rodiyati Azrianingsih, Muhamad Agus Wibowo, Yuli Arif Tribudi, Diah Wulandari Rousdy, Didik Wahyudi, Ayu Tri Agustin, Nashi Widodo
Exploring the natural product as a potential alpha-amylase inhibitor with no adverse side effects is still being concerned in developing diabetes mellitus drugs. This study aims to identify the phytochemical components of porang tuber extract. We also investigated bioactivity and predicted the biological function of the porang tubers bioactive in inhibiting human pancreatic alpha-amylase. The phytochemical components were analyzed by Liquid Chromatography Mass Spectrophotometry (LCMS). We investigated the biological activity of compounds as anti-diabetic agents by the PASS server. The 3D structure of the active compound that was showing anti-diabetic activity was prepared using PyRx software. Docking simulations were analyzed using Hex 8.0 software, while for visualization Discovery Studio Client 4.1 software was used. Our study found the presence of 67 phytochemical constituents in porang tuber extract. The five largest compounds with anti-diabetic activity are glucomannan, mannan, and flavonoids (quercetin, orientin, and hyperoside). PASS analysis showed that glucomannan and mannan had the most potential as anti-diabetic. It was determined by the Pa value of 0.77. The active sites of alpha-amylase (ASP197, GLU233, and ASP300 residues) were bound by glucomannan, mannan, orientin, and hyperoside, which plays an essential role in catalytic activity. Glucomannan showed the strongest interaction with the pancreatic alpha-amylase domain (lowest binding energy -394.1 kcal/mol). This result indicates that porang tubers are very potential as an alpha-amylase inhibitor. Further research is needed to validate this finding. © (2024), (Hashemite University). All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Indonesia; Porang Research Center (PRC) Brawijaya University, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Indonesia; Department of Animal Science, Faculty of Agriculture, Tanjungpura University, Indonesia; Biology Department Universitas Islam Negeri Maulana Malik Ibrahim Malang, Indonesia; Medical Laboratory Technology Study Program, Dr. Soebandi University, Indonesia