Mohamad Raviansyah Jawindra, Widjiati Widjiati, Rimayanti Rimayanti, Suherni Susilowati, Epy Muhammad Luqman, Mirni Lamid, Viski Fitri Hendrawan
| Kacang goats, native to Indonesia, play a vital role in enhancing food security and supporting rural economies. Nonetheless, their population and genetic quality are still not at an optimal level. In vitro maturation (IVM) plays a crucial role in genetic conservation, by impacting oocyte competence and embryonic development. This research assessed the impact of insulin-like growth factor-1 (IGF-1) supplementation in the maturation medium on the expression of TGF-β and GDF-9 in Kacang goat oocytes. Oocytes were harvested from goat ovaries and subjected to in vitro maturation with IGF-1 at concentrations of 0 (control), 50, 100, and 150 ng/mL. Immunocytochemistry evaluated the expression of TGF-β and GDF-9. Oocytes exposed to 100 ng/mL IGF-1 exhibited a marked increase in the expression of TGF-β and GDF-9 compared to other groups (p < 0.05). A significant positive correlation exists between TGF-β and GDF-9 (r = 0.499; τ = 0.424; p < 0.05). The results indicate that 100 ng/mL IGF-1 effectively promotes oocyte maturation through the activation of signaling pathways. In summary, administering 100 ng/mL IGF-1 is advised to enhance oocyte quality in Kacang goats, which may lead to improved IVF outcomes. Copyright: 2025 by the authors. Licensee ResearchersLinks Ltd, England, UK. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Reproductive Biology, Universitas Airlangga, Surabaya, Indonesia; Division of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia; Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia; Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia; Faculty of Veterinary Medicine, Brawijaya University, Malang, Indonesia
