Dian Ratnawati, Gatot Ciptadi, Sri Rahayu, Dita Retnowulan, Fardha Ad Durrun Nafis, Taufiq Ridwan, Achmad Setiyono, Iswati Iswati, Yuli Arif Tribudi, Sulistiyoningtiyas Irmawanti, Trinil Susilawati
This research aimed to evaluate the effectiveness of Caudal Epididymis Plasma (CEP) combined with nanoparticle soybean extract (NSE) as diluents in maintaining the quality, motility and DNA integrity of Bali cattle spermatozoa in liquid semen. The study utilized semen from Bali cattle and employed a Randomized Complete Block Design (RCBD). Four treatment groups were tested: T1 (CEP + 10% Egg Yolk), T2 (CEP + 40% NSE), T3 (CEP + 50% NSE) and T4 (CEP + 60% NSE). The observed parameters included sperm motility, viability, abnormalities, membrane integrity, Superoxide Dismutase (SOD), Malondialdehyde (MDA), DNA fragmentation and Scanning Electron Microscope (SEM) morphology. Motility, viability, abnormalities and membrane integrity were analyzed using ANOVA via SPSS 16, while SOD, MDA and DNA fragmentation were assessed using Microsoft Excel; SEM results were interpreted descriptively. The findings revealed that T4 had significantly higher abnormalities (18.81%) compared to other groups (p < 0.05). However, T3 and T4 showed superior progressive motility on day 9 of cold storage. Higher SOD and MDA levels were found in T1 on day 7. DNA fragmentation remained low across all treatments, ranging from 3.65% to 5.35%. The use of CEP with 50-60% NSE effectively preserved semen quality for up to nine days of storage. © 2025 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Research Center for Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency of The Republic of Indonesia (BRIN), Cibinong Sciences Center, Bogor, Indonesia; Department of Animal Reproduction and Breeding, Faculty of Animal Science, Universitas Brawijaya, Malang, Indonesia; Department of Biology, Faculty of Mathematic and Natural Science, Universitas Brawijaya, Malang, Indonesia; Singosari National Artificial Insemination Center, Malang, Indonesia; Politeknik Pembangunan Pertanian, Malang, Indonesia; Departement of Animal Science, Faculty of Agriculture, Universitas Tanjungpura, Pontianak, Indonesia
