Khairul Anuar Shariff, Alexander Patera Nugraha, Ahmed Hafedh Mohammed Mohammed, Ida Bagus Narmada, I Gusti Aju Wahju Ardani, Nastiti Faradilla Ramadhani, Ratri Maya Sitalaksmi, Wibi Riawan, Helen Susilowati, Suryo Kuncorojakti, Bramasta Nugraha, Guang Hong
Elucidate the dicalcium phosphate dihydrate (DCPD)-coated-beta-tricalcium phosphate (β-TCP) porous granules osteoinductivity performance in adipose-derived mesenchymal stem cells (ADMSCs). β-TCP porous was made by adding the sodium chloride (NaCl) powders with the dry powder of DCPD and CaCO3 in the concentrations 40:60 (by wt%). Then, DCPD layer will be formed on porous β-TCP granules by immersing the granules in an acidic calcium phosphate solution. ADMSCs cultured was used to examine the biocompability of 300–600 µm and 600–1,000 µm DCPD-coated-porous β-TCP by means of microculture tetrazolium technique (MTT assay) and scratch assay. Bone morphogenic protein-2 (BMP-2), Osterix, Runt-related transcription factor-2 (Runx2), Alkaline phosphatase (ALP), Osteopontin, Osteonectin expression were investigated. The DCPD-coated porous β-TCP granules were culture together with ADMSCs is biocompatible. BMP-2, Runx2, Osterix, ALP, Osteopontin, Osteonectin expression in ADMSCs after culture together with DCPD-coated-β-TCP significantly enhanced. DCPD-coated-porous-β-TCP granules have good biocompatibility and high osteoconductivity in ADMSCs. © 2025, Japanese Society for Dental Materials and Devices. All rights reserved.
Biomaterials Niche Area, School of Materials and Mineral Resources Engineering, Universiti Sains Malaysia, Engineering Campus, Pulau Pinang, Nibong Tebal, 14300, Malaysia; Orthodontic Department, Faculty of Dental Medicine, Universitas Airalngga, Jl. Prof. Dr Moestopo No. 47, East Java, Surabaya, 60132, Indonesia; Division for Globalization Initiative, Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, Miyagi, Sendai, 980-0872, Japan; Dentomaxillofacial Radiology Department, Faculty of Dental Medicine, Universitas Airalngga, Jl. Prof. Dr Moestopo No.47, East Java, Surabaya, 60132, Indonesia; Prosthodontic Department, Faculty of Dental Medicine, Universitas Airalngga, Jl. Prof. Dr Moestopo No.47, East Java, Surabaya, 60132, Indonesia; Biochemistry Biomolecular Laboratory, Faculty of Medicine, Brawijaya University, East Java, Malang, Indonesia; Research Center for Vaccine Technology and Development, Institute of Tropical Disease, Campus C, Jl. Mulyosari, East Java, Surabaya, 60115, Indonesia; Stem Cell Biology, Novo Nordisk, Copenhagen, Denmark
