Suherni Susilowati, Imam Mustofa, Zulfi Nur Amrina Rosyada, Tri Wahyu Suprayogi, Tatik Hernawati, Adeyinka Oye Akintunde, Yudit Oktanella, Aswin Rafif Khairullah, Ulvi Fitri Handayani, Riza Zainuddin Ahmad, Lili Anggraini, Bima Putra Pratama, Chairdin Dwi Nugraha, Latifah Latifah
Background: One native goat species in Indonesia that faces extinction because of cross-breeding is the Kacang goat (Capra hircus). In addition to ensuring food security as a meat-producing livestock, artificial insemination of the Kacang goat could simultaneously conserve it and improve its genetic quality. Aim: The purpose of this study was to investigate the effect of the addition of epigallocatechin-3-gallate nanoparticles (EGCG) to skimmed egg yolk (SM-EY) extender on the quality of post-thawed Kacang buck spermatozoa for artificial insemination. Methods: Fresh semen of Kacang buck was diluted in SM-EY without (T0) and with the addition of 50 or 100 mg EGCG/dl extender for T1 and T2, respectively. Extended semen was frozen in liquid nitrogen (−196°C) for a week in French tiny straws with 60 million spermatozoa per straw, following a standard protocol. Six thawed replicates from each group were tested for sperm motility, viability, intact plasma membrane (IPM), malonaldehyde (MDA), DNA integrity, and chromatin density. Results: Pre-equilibration sperm motility, viability, and IPM were not significantly different among the groups (p > 0.05). Post-equilibration sperm motility, viability, and IPM were higher (p < 0.05) in the EGCG group than in the control group. In post-thawed semen, the EGCG groups showed higher sperm motility, viability, IPM, and DNA integrity and lower MDA levels (p < 0.05) than the control group. The chromatin density did not differ between the groups (p > 0.05). Conclusion: The addition of 50 or 100 mg EGCG/dl SM-EY extender significantly improved the post-thawed spermatozoa quality of Kacang buck. © 2025, Faculty of Veterinary Medicine, University of Tripoli. All rights reserved.
Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia; Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Agriculture and Industrial Technology, Babcock University, Ilishan Remo, Nigeria; Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia; Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Bogor, Indonesia; Research Center for Animal Husbandry, National Research and Innovation Agency (BRIN), Bogor, Indonesia; Research Center for Agroindustry, National Research and Innovation Agency (BRIN), South Tangerang, Indonesia
