Imam Mustofa, Suherni Susilowati, Tri Wahyu Suprayogi, Adeyinka Oye Akintunde, Yudit Oktanella, Djoko Agus Purwanto
Background and Aim: The Kacang goat (Capra hircus) is an indigenous livestock species in Indonesia that is at risk of extinction due to cross-breeding. Artificial insemination (AI) techniques are expected to increase the population of these goats. This study aimed to determine the addition of epigallocatechin-3-gallate chitosan nanoparticles (EGCG CNPs) to skim milk–egg yolk (SM–EY) extender to obtain the best possible quality of post-thawed Kacang buck semen for AI. Materials and Methods: Fresh Kacang buck semen was diluted in SM–EY without or with the addition of 0.5, 1.0, 1.5, or 2.0 µg of EGCG CNPs/mL extender. Extended semen was packaged in French mini straws, frooze, and stored in liquid nitrogen at −196℃ for 24 hours. Six replicates from each treatment group were thawed for catalase, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, malondialdehyde (MDA), sperm intact plasma membrane (MPI), living cells and motility analyses. Results: Post-thawed semen that was previously frozen without EGCG CNPs in the extender (control group) exhibited the lowest levels of catalase, DPPH, sperm viability, sperm motility, IPM, and the highest levels of MDA. However, the addition of EGCG CNPs at doses of 1.5 µg/mL extender increased post-thawed catalase, DPPH, sperm IPM, viability, and sperm motility and decreased MDA levels (p < 0.05) than those of control group. Conclusion: This study was the first in which EGCG CNPs were used in SM–EY extender, and the addition of only 1.0 µg/mL of EGCG CNPs in this extender increased the antioxidant capacity and post-thawed quality of Kacang buck semen. Copyright: © 2025 Mustofa I et al.
Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Airlangga University, East Java, Surabaya, 60115, Indonesia; Department of Agriculture and Industrial Technology, Babcock University, Ogun State, Ilishan-Remo, Nigeria; Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Brawijaya University, East Java, Malang, Indonesia; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Airlangga University, East Java, Surabaya, 60115, Indonesia
