Moh Dliyauddin, Firda Nuri Asyhari, Heni Sukma Zulfatim, Nenis Try Melani Putri, Ahmad Shobrun Jamil, Aris Soewondo, Muhammad Halim Natsir, Mansur Ibrahim, Sri Rahayu, Muhammad Sasmito Djati, Muhaimin Rifa’i
This study aimed to assess the impact of administering Cheral® (Curcuma longa and Phyllanthus niruri) on the expression of T lymphocytes and regulatory T cells. The research was conducted in vivo using 24 healthy female Wistar rats, which were categorized into four treatment groups: normal (control), dose 1 (156.25 mg/Kg BW), dose 2 (312.5 mg/Kg BW), and dose 3 (468.75 mg/Kg BW). The rats were orally administered Cheral® for a period of 90 days. T lymphocytes and regulatory T cell profiles were analyzed using flow cytometry. The data obtained were subsequently subjected to analysis using SPSS software with One-way ANOVA and Post hoc tests (p-value <0.05). The profiles of T lymphocytes and regulatory T cells following Cheral® administration exhibited diverse outcomes when compared to the normal group. Specifically, dose 2 led to a significant increase in CD4, while dose 1 resulted in a significant increase in CD8. However, the profile of regulatory T cells after Cheral® administration did not exhibit any significant differences when compared to the control group. In conclusion, this research indicates that the compounds present in Cheral® have the potential to modulate the immune system through their effects on T lymphocytes and regulatory T cells. © (2025), (Slovak University of Agriculture). All Rights Reserved.
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, 65145, Indonesia; Faculty of Animal Life Science, Brawijaya University, Malang, 65145, Indonesia; Faculty of Pharmacy, Megarezky University, Makassar, 90234, Indonesia
