Hefti Salis Yufidasari, Aji Sutrisno, Agustin Krisna Wardani, Teti Estiasih
Lipase (triacylglycerol acylhydrolase, EC3.1.1.3) is an enzyme that has an important role in modern biotechnology. Lipase is known to have high activity in hydrolysis reactions and in synthetic chemistry. Lipase can act as a biocatalyst for hydrolysis, esterification, alcoholysis, acidolysis and aminolysis reactions. Lipase hydrolyzes triglycerides into free fatty acids, partial glycerides, and glycerol. Lipase is an enzyme that is widely distributed in nature and is produced by several plants, animals and microorganisms which has quite large potential physiologically and industrially. Isolate is a bacterial isolate obtained from fish egg, samples stored at cold temperatures, where the isolate is thought to be capable of producing enzymes that have high lipolytic activity at low temperatures. The purpose of this research was to characterize the bacterial isolate that produces the lipase enzyme, namely testing enzyme activity and molecular identification to estimate bacterial species. The results showed that isolate TL2510B had the highest enzyme activity at a temperature of 25°C of 141.33 U/mL, the enzyme activity based on pH showed the highest at pH 10, namely 106.00 U/mL, while in optimization the highest enzyme production was obtained at the 24th hour, of 104.69 U/mL. Identification of the isolate based on 16s rRNA analysis showed 97.02% similarity to Morganella morganii strain F6C1 (accession number JX999959.1). © Published under licence by IOP Publishing Ltd.
Faculty of Agricultural Tecnology, Universitas Brawijaya, Malang, Indonesia; Faculty of Fisheries and Marine Science, Universitas Brawijaya, Malang, Indonesia
