Muhamad Amin, Karina Kristanti Ekarani Rahardjo, Alief Erwaanda Panosa, Woro H. Satyantini, Akhmad Taufiq Mukti, Muhamad Ali, Laila Musdalifah, Halil Akhyar, Uun Yanuhar, Mohammad Noor Amal Azmai, Takaomi Arai
The etiological agent of white feces disease (WFD) infecting Pacific white shrimp Penaeus vannamei in Indonesia farms remains obscure. The present study aimed to identify possible causative agents of WFD infection in Pacific white shrimps cultured in Indonesian farms. WFD-infected and healthy samples (shrimp gut and rearing water) were collected from 8 commercial shrimp farms in East Java, Indonesia followed by bacterial community profiling using HiSeq sequencing of 16S rRNA gene amplicons. The results showed that the microbiota composition in the guts of WFD-infected shrimps was significantly different (p < 0.05) from the guts of healthy shrimps in term of genus and bacterial species. The intestinal bacterial communities of WFS-infected shrimps were overrepresented by Vibrio coralliilyticus, whereas Paracoccus was underrepresented. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States functional predictions indicated that relative abundances of 12 genes associated with the pathogenicity of bacteria including amino acid metabolisms, carbohydrate metabolisms, glycan biosynthesis, and xenobiotic biodegradation and metabolism were significantly (p < 0.05) higher in the gut microbiota of WFD-infected shrimps. These findings provide insights into the microbiome domination and their metabolic activities in the digestive tract of WFD-infected shrimps and suggest that V. coralliilyticus is a possible causative agent of WFD in cultured Indonesia Pacific white shrimp. © 2025 Elsevier Ltd
Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Campus C, Jl Mulyorejo, Mulyorejo, East Java, Surabaya, 60115, Indonesia; Master of Fisheries Science, Faculty of Fisheries and Marine, Universitas Airlangga, Campus C, Jl Mulyorejo, Mulyorejo, East Java, Surabaya, 60115, Indonesia; Faculty of Animal Science, University of Mataram, Jl Majapahit No. 62, West-Nusa Tenggara, Mataram, 83115, Indonesia; Research Centre for Fishery, National Research and Innovation Agency, Jl Raya Jakarta-Bogor KM. 47, Nanggewer Mekar, Cibinong, West Java, Bogor, 16911, Indonesia; Informatics Engineering Study Program, Faculty of Engineering, University of Mataram, Jl Majapahit No. 62, West-Nusa Tenggara, Mataram, 83115, Indonesia; Faculty of Fisheries and Marine Science, Brawijaya University, East Java, Indonesia; Department of Biology, Faculty of Science, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia; Aquatic Animal Health and Therapeutics Laboratory, Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia; Environmental and Life Sciences Program, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, BE1410, Brunei Darussalam
