Sefihara Paramitha Sakti, Fikriya Novita Sari, Farida Rachmawati, Sri Widyarti, Muhaimin Rifa'i
This study aims to investigate the effectiveness combination of Phyllanthus niruri and Catharanthus roseus extract (PCE) in modulating oxidative stress based on the levels of MDA, superoxide dismutase SOD, and Nrf2. Experimental animals were divided into six different groups- healthy control (normal), cancer (DMBA), cancer mice with cisplatin administration, cancer mice with PCE administration with three different doses, including dose 1 (500 mg/kg PN+15 mg/kg CR), dose 2 (1000 mg/kg PN+75 mg/kg CR), and dose 3 (2000 mg/kg PN+375 mg/kg CR). The mice were injected with DMBA once a week for six weeks to induce cancer in mice. PCE was orally administrated to breast cancer mice for 14 days. The levels of MDA, Nrf2, and SOD were analyzed by flow cytometry. The results showed that breast cancer induction using DMBA increased the level of MDA and Nrf2 but decreased the level of SOD significantly compared to the normal group (p<0.05). In certain doses, administration of PCE could reduce MDA and Nrf2 levels and increase the level of SOD significantly (p<0.05). The PCE has potency as an alternative therapeutic agent to modulate redox homeostasis in breast cancer by suppressing MDA and Nrf2 levels, also increasing SOD levels. © 2025 American Institute of Physics Inc.. All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, 65145, Indonesia
