Muhammad Maskur, Asep Awaludin Prihanto, Muhamad Firdaus, Rahmi Nurdiani
Caulerpa (C.) cylindracea is a species of green seaweed known for its antibacterial bioactivity. Crude extracts of weeds are promising to way to check their antimirobiolal potential against various pathogens. Alongwith other factors, the bioactivity of weed extracts also greatly dependent upon method of its extraction. Consequently, this study aimed to identify the bioactive constituents and antibacterial bioactivity of crude extracts of C. cylindracea obtained through the ultrasound-assisted extraction (UAE) method against Morganella morganii bacteria. The C. cylindracea was extracted using UAE with different methanol and acetone ratio and various time. Best extract against M. morganii would be further identified its bioactives using LC-HMRS. A15-minute extraction time, a maximum temperature of 40°C, a frequency of 50 Hz, an amplitude of 50%, and a solvent ratio of methanol to acetone (2.5:7.5) in the stolon, showed best antibacterial activity. A total of 24 bioactive compounds is identified, among which three exhibit antibacterial activity: betaine, 5-Fluoro-2-hydroxybenzoic acid, and Isoamylamine. Their inhibitory mechanisms involve the disruption of bacterial membranes and metabolic processes, ultimately leading to cell death. © 2025, Unique Scientific Publishers. All rights reserved.
Doctoral Program, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, East Java, Malang, 65145, Indonesia; Department Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, East Java, Malang, 65145, Indonesia; Polytechnic of Marine and Fisheries Bone, Jl. Sungai Musi Km.9, Bone, South Sulawesi, 92718, Indonesia
