Diana Aisyah, Ayu Winna Ramadhani
This study aimed to determine the effectiveness of homologous β-actin promoter concentration from the common carp in expressing the GFP (Green Fluorescent Protein) marker gene during the transgenesis process of koi fish using electroporation and SMGT (Sperm Mediated Gene Transfer) methods on sperm, embryos, and larvae of Koi fish. The research employed an experimental method using a completely randomized design (CRD), with three concentrations of cβa-GFP: 10, 30, and 50ng/ μl.The electroporation treatment was conducted using an electric voltage of 30 volts and four pulses. The observed parameters include the fluorescence and integration region of cβa-GFP in sperm, eggs, and larvae, as well as the identification of GFP in embryos and larvae, and sperm quality. The results showed that the use of the cβa (Cyprinus carpio β-actin) promoter successfully expressed GFP marker gene fluorescence in sperm, embryos, and larvae of koi fish, with the best concentration being 10ng/ μl. © 2025, Egyptian Society for the Development of Fisheries and Human Health. All rights reserved.
PSDKU Aquaculture, Department of Fisheries and Marine Resources Management, Faculty of Fisheries and Marine Science, University of Brawijaya, Indonesia
