Dian Siswanto, Nurul Istiqomah, Azizuddin Muhammad Nashafi, Mukhaddam Muhammad, Fathul Mukaromah, Irfan Mustafa
The study investigated bacterial isolation from lead-contaminated soil, revealing the lower bacterial density compared to agricultural soil bacteria, indicating soil degradation. Among the isolated bacteria, three isolates with codes L03, L16 and L19 exhibited high tolerance to lead concentrations up to 1,500 mg/L. Selected isolates demonstrated the ability to produce Indole-3-Acetic Acid (IAA) hormone, with one strain notably producing the highest IAA concentration. Furthermore, three isolates exhibited significant lead bioaccumulation efficiency. Molecular identification revealed Lysinibacillus fusiformis as the highest IAA-producing strain and lead accumulator. Plant growth experiments analysed the bacteria’s potency to alleviate the heavy metal stress on Codiaeum variegatum, Dracaena reflexa and Jasminum humile. Additionally, bacterial addition decreased lead absorption only by D. reflexa, potentially through biosorption and bioaccumulation mechanisms. Integrating L. fusiformis into phytoremediation strategies could offer an effective and sustainable approach for remediating Pb-contaminated environments. © Penerbit Universiti Sains Malaysia, 2025.
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, 65145, Indonesia; National Research and Innovation Agency of The Republic of Indonesia (BRIN), Jakarta, 10340, Indonesia; Center for Crop Seedling and Plant Protection (BBPPTP) of Surabaya, Jombang, 61482, Indonesia
