A.P.A. Yekti, J. Riyanto, C. Hanim, R. Prafitri, G. Ciptadi, S. Rahayu, T. Susilawati
Background: Beef cattle fattening business requires male cattle because they grow faster so that by using sexed semen (Y sperm) it is expected to get a male calf. The purpose of this study was to determine the success of artificial insemination using non-sexed and sexed frozen semen using percoll density gradient centrifugation (PDGC) method. Methods: A total of 120 female Limousin Crossbred were used in this study. The cows were raised on smallholder farms aged between 3-9 years with a body condition score (BCS) between 3-7 (score 1-9). The treatment was T0: 60 cows were inseminated using non-sexed frozen semen while T1: 60 cows were inseminated using sexed Y frozen semen. The insemination was performed with double doses at the 2nd and 8th hours after oestrus and deep insemination. The parameter measured were NRR-1, NRR-2, conception rate and pregnancy rates. Determination of pregnancy was performed using rectal palpation and ultrasonography (USG). Result: The results showed that NRR-1 and NRR-2 at T0 were 47 (78.33%) and 42 (70%), while at T1 were 37 (61.67%) and 35 (58.33%), respectively. While conception rate at T0 and T1 was 22 (36%) and 18 (30%), pregnancy rate at T0 and T1 was 31 (56%) and 19 (31.67%) and silent heat at T0 and T1 was 24 (40%) and 26 (43.3%). © 2025, Agricultural Research Communication Centre. All rights reserved.
Faculty of Animal Science, Universitas Brawijaya, Malang, Indonesia; Faculty of Agriculture, Universitas Sebelas Maret, Solo, Indonesia; Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia; Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang, Indonesia
