Ardiansyah Kurniawan, Anik M. Hariati, Andi Kurniawan, Nia Kurniawan, Wahyu E. Kusuma, Dewa GR Wiadnya
Osteochilus spilurus (Bleeker, 1851), with a vast distribution in Sundaland, is one of the least utilized freshwater species. The lack of interest in this species has resulted in restricted human-caused dispersal and has offered the opportunity for a precise phylogeographic study. Fish samples were caught from six rivers on the islands of Bangka, Belitung, and Kalimantan. Fish identification was conducted using DNA barcoding of cytochrome oxidase 1 with Fish-F1 and Fish-R1 primers. Twenty-five 655 bp nucleotide sequences from this research are recorded in NCBI and represent the first such records. Bangka-Belitung and Kalimantan samples form intraspecific relationships within each region but are separated into different clades between the two regions. This separation suggests that O. spilurus may represent a cryptic species due to historical changes in the formation of the Karimata Strait between the two regions. O. spilurus has the potential to be used as a distribution and phylogeographic model for freshwater fish in Sundaland. © 2025, Egyptian Society for the Development of Fisheries and Human Health. All rights reserved.
Department of Aquaculture, Faculty of Agriculture, Fisheries, and Marine Science, Universitas Bangka Belitung, Indonesia; Faculty of Fisheries and Marine Science, Universitas Brawijaya, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Indonesia
