Titiek Indhira Agustin, Happy Nursyam, Muhammad Firdaus, Muhaimin Rifa’i
The utilization of marine byproducts, such as shark cartilage, presents a sustainable strategy for the development of novel biomedical applications. This study investigated the anti-inflammatory potential of shark cartilage extract derived from Prionace glauca, a byproduct of fisheries, in a lipopolysaccharide (LPS)-induced mouse model of inflammation. The cartilage was processed via maceration in distilled water (1:10 b/v) at 45°C for 8 hours, and its chondroitin sulfate (CS) content was quantified using High-Performance Liquid Chromatography (HPLC), yielding an average of 2.23%. Male BALB/c mice were assigned to seven groups, including an LPS-only group, a standard CS group, a commercial supplement group (Welmove), and three dosage groups of shark cartilage extract (50, 100, and 200%). Inflammation was induced through intraperitoneal injection of LPS (1mg/ 100mL PBS), and immune responses were assessed by analyzing TNF-α and IFN-γ expression in CD4+ T cells and NK cells via flow cytometry. The extract significantly reduced the percentages of CD4⁺TNF-α⁺, CD4⁺IFN-γ⁺, and NK⁺IFN-γ⁺ cells in a dose-dependent manner (P < 0.05), with higher doses (D2 and D3) restoring cytokine expression toward baseline levels. These findings highlight the potential of shark cartilage byproducts as a valuable source of natural anti-inflammatory agents, supporting their further development in pharmaceutical and biomedical fields. © 2025, Egyptian Society for the Development of Fisheries and Human Health. All rights reserved.
Fisheries and Marine Science Graduate Program, Faculty of Fisheries and Marine Science, University of Brawijaya, Jl. Veteran Malang, East Java, 65149, Indonesia; Fisheries Study Program, Faculty of Engineering Marine Science, University of Hang Tuah, Jl. Arief Rahman Hakim 150, Surabaya, 60111, Indonesia; Department of Aquatic Product Technology, Faculty of Fisheries and Marine Science, University of Brawijaya, Jl. Veteran Malang, East Java, 65149, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang, Indonesia
