S.P. Sakti, P. Hauptmann, B. Zimmermann, F. Bühling, S. Ansorge
The application of quartz crystal microbalance (QCM) as affinity immunosensor of human serum albumin (HSA) has been investigated. The aim of the investigation was the development of a sensor for simple use for physicians in practice. The HSA immunosensors were constructed as a disposable immunosensor based on a cost effective QCM sensor. A self mixing cell without pumping system was constructed together with the QCM-immunosensor for easy handling. The HSA antibody was immobilized on top of a quartz resonator coated with a mixture of polystyrene and p-tert-butyl-calix[8]arene. There were no significant differences between the measured maximum frequency change of off-line measurement in air and on-line measurement in PBS. In this experiment, the developed immunosensor allows the measurement of HSA in PBS in the concentration range from 1 to 250 μg/ml. The limit value concentration of 20 μg/ml between ill and healthy condition can be well measured. The sensor shows a selectivity to HSA in urine. © 2001 Elsevier Science B.V. All rights reserved.
Institute for Micro and Sensor System, Otto von Guericke University Magdeburg, 39016 Magdeburg, P.O. Box 4120, Germany; Insitute of Immunology, Otto von Guericke University Magdeburg, 39120 Magdeburg, Leipziger-Street 44, Germany; Department of Physics, Brawijaya University MT Haryono 169, Malang 65145, Indonesia
