Dyah Kinasih Wuragil, Aulanni'am
Diabetes Mellitus (DM) is a metabolic disease caused by perturbants of insulin production which has function to maintain blood glucose to muscle and fatty tissue. Type 1 DM or Insulin Dependent Diabetes Mellitus (IDDM) result from chronic autoimmune destruction of pancreatic β cells and cause insulin deficiency. According by experimental of many diabetisian, show that extract of Andrographis paniculata's leaves, can use to treat of DM. The aims of these researches are to know the prospect of A. paniculata for declining of blood glucose levels, also the presenting of TNFα in pancreas sectioning. Induction of DM in rat (Rattus norvegicus Wistar strain) 1,5-2 months were conducted by injecting Multiple Low Dosage Streptozotocin (MLD-STZ) intraperitonially (i.p) at dosage of 15 mg/Kg BW in 5 days. The treatments divided in 3 groups were (I): negative control, (II): positive control, that consist of diabetic rats, and (III): diabetic rats treated with 3-5 ml A.paniculata's extract everyday in a week. Histological observation and measurement of blood glucose level were conducted in preimmune, after STZ injection and after treatment of A. paniculata. These results provided that A.paniculata's leaves extract have the effect for declining of blood glucose level and the presenting of TNFα in pancreas showed overproduction of TNFα cause inflamatoty in diabetic rats.
Veterinary Medicine School, Brawijaya University, Malang, Indonesia; Biochemistry Laboratory, Department of Chemistry, Faculty of Life Sciences, Brawijaya University, Malang, Indonesia
