Ahmad Fauzi, Iwan Setiawan, Fitri Ariyanti
The aim of this research was to identify the role of Virgin coconut oil (VCO) in treatment of infections caused by Staphylococcus aureus which were examined by histopathologic pictures of the heart, lungs and skin. This study used 25 female mice of Swiss strain, 4 weeks old with average weight of 23 grams. The mice were randomly divided into five groups, each group was consisted of 5 mice. Group I (K 1) as a control, mice were given phosfat buffered saline (PBS) 0.05 ml per oral (po). Group II (K2) mice were infected by Staphylococcus aureus 1 × 10 2 bacteria/ml via intraperitoneal (IP) at the 1 st day and were given 0.05 ml PBS. Group III (K3) mice were infected by Staphylococcus aureus IP at the 1 st day, then in the 3 rd day mice were given VCO 0.05 ml per oral (po) until 1 week. Group IV (K4) mice were infected by Staphylococcus aureus topically at the 1 st day. Group V (K5) mice were infected by Staphylococcus aureus topical at the 1 st day and then in the later day the mice were given VCO by topical application until 1 week. In the 4 th week, mice which were infected by Staphylococcus aureus via intraperitoneal (Group II and III) were necropsied for histopathology of liver and lungs observation. While, skin of mice which were infected by Staphylococcus aureus Group IV and V) were necropsied at the 3 rd, 6 th and 9 th day to examine the histopathologic of skin. The results showed that Staphylococcus aureus infection caused infiltration of inflammatory cells (polymorphonuclear cells) in the liver and necrosis of hepatocytes, granulomatous inflammation in lung, narrowing the pulmonary septa alveoli and skin necrosis. Mice which were infected by Staphylococcus aureus then treated with VCO have ability to repair their liver tissue, lung and skin from damage caused by Staphylococcus aureus infection.
Faculty of Veterinary Medicine, University of Brawijaya, Malang 65145, Haryono st 169, Indonesia; Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta 55281, Fauna 2 st, Indonesia
