Umi Marwati, Keiko Funasaka, Koki Toyota, Arata Katayama
Microbial communities in a soil with different fertilizing practices were characterized based on the utilization pattern of 26 aromatic compounds as single substrates, which are considered to occur in the soil environment. Their utilization was detected by the dehydrogenase reaction with p-iodonitrophenyltetrazolium violet (INT) after 72 h or 7 d of incubation at 22°C. The compounds involved in the metabolic pathways of p-coumarate, hippurate, m-hydroxybenzoate, and vanilate were utilized in all the soils. o-Coumarate, p-methoxy cinnamate, and phenol were utilized only in the soils amended with farmyard manure, indicating the wider spectra of the compounds utilized. Inhibition by cycloheximide showed that fungi were essential in the utilization of mandelate, ²-resorcylate, phthalate, and terephthalate in the soils used and bacteria in the utilization of hippurate. © 2003 Taylor & Francis Group, LLC.
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8603, Japan; Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, 184-8588, Japan; Research Center for Advanced Waste and Emission Management, Nagoya University, Nagoya, 464-8603, Japan; Faculty of Mathematics and Natural Sciences, Brawijaya University, East Java, Indonesia
