Cynthia Setiawan, Hari Purnomo, Joni Kusnadi
Microwave-Assisted Extraction is a novel kind of extraction method that recently used for herbs and spices. Many studies have shown that the irradiative heating mechanism of microwave works effectively throughout the medicinal plant cell. Teak (Tectona grandis) is a native and common tropical tree from Indonesia. Its timbers have become the main commodity of forestry industry in Madiun (East Java). Recently, its leaves are known to possess antioxidant activity. Thus, this method was expected to increase the activity of Teak leaves antioxidant. This research highlighted a qualitative phytoconstituents screening on Teak leaves. The aim of this in-vitro study was to compare the effectivity and efficiency of extraction method (soxhletation and Microwave-Assisted Extraction) towards Teak leaves antioxidant. The examination of total phenol content by Folin-Ciocalteau method; DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging activity; electro-donating activity by Ferric-Reducing Antioxidant Power assay; and hydrogen peroxide scavenging activity of Teak leaves antioxidant were carried out in respect to show the significance of extraction method towards the antioxidant activity. The utilization of microwave during extraction could increase the yield and concentration of phenolic compound in Teak leaves extract, further affects its antioxidant activity. Microwave-Assisted Extraction exhibited higher antioxidant activity than soxhletation.
Department of Food Science and Technology, University of Brawijaya, Malang, East Java, Indonesia; Department of Animal Husbandry, University of Brawijaya, Malang, East Java, Indonesia
