Uun Yanuhar, Ery Gusman, Diana Arfiati
The main topic of this study are to find out of the proliferation and expression of IFN-γ and NF-κB cell to Humpback grouper that exposured by immunogenic protein of Viral Nervous Necrosis (VNN). The methods used on this study were exploration by doing isolation to Humpback grouper organs both normal and infected by immunogenic protein of VNN coat protein isolation protein fractionation by SDS-PAGE separation and cutting of protein bands from SDS-PAGE result coat protein measured by Nano drop spectrophotometer Hemagglutination (HA) Test of VNN immunogenic protein and IFN-γ and NF-κB cell proliferation and expression qualitative detection by Immunohistochemistry (IHC).The results of this research confirm that HA test was positive. It showed that 459 kDa VNN immunogenic protein had been reacted by agglutination reaction between 45.9 kDa VNN immunogenic protein and erythrocytes of humpback grouper with sensitivity of 0015625. Positive concentration result was up to 1/64 or its sensitivity of 0.015625. It means antigen determinant (epitope) from immunogenic protein have highly sensitivity from range 0.006 to 0.06. IHC test result show that 459 kDa VNN protein immunogenic epitope have ability to induce humpback grouper tissue receptors by creating immune cell qualitatively there are IFN γ and NF-κB cell immune to humpback grouper specific tissue organ eye brain and kidney after labeled by secondary antibody anti IFN γ and NF-κB cell anti mouse. ELISA test using the secondary antibody that same with examination test of IHC were showed that proliferation and expression of IFN γ and NF-κB cell quantitatively had been confirmed value positive around 0.9 mg/ml concentration. The conclusion of this studies showed that immunogenic protein of VNN with weight molecule of 45.9 kDa have ability to induce proliferation and expression of immune cells of humback grouper as like IFN γ and NF-κB cells both qualitative and quantitative on tissue organ especially eye brain and kidney organ.
Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia; Faculty of Fisheries, Borneo University, East Kalimantan, Indonesia
