Dae Sik Jang, Hye-Young Min, Moon-Sun Kim, Ah-Reum Han, Tri Windono, Gwang-Ho Jeohn, Sik Kang Sam, Sang Kook Lee, Eun-Kyoung Seo
A new humulene sesquiterpene, 5-hydroxyzerumbone (5-hydroxy-2E,6E,9E- humulatrien-8-one) (1) and a known compound, zerumboneoxide (2) were isolated from the rhizomes of Zingiber zerumbet (Zingiberaceae), and found to inhibit lipopolysaccharide-induced nitric oxide production in murine macrophage RAW 264.7 cells with IC50 values of 14.1 and 23.5 μM, respectively, by bioassay-guided fractionation (positive control: Nω-monomethyl-L- arginine, IC50=21.3 μM). The structure of 1 was determined by spectroscopic methods including 1D and 2D-NMR. © 2005 Pharmaceutical Society of Japan.
Division of Molecular Life Sciences, Ewha Womans University, Seoul 120-750, 11-1 Daehyun-dong, Seodaemun-gu, South Korea; College of Pharmacy, Ewha Womans University, Seoul 120-750, 11-1 Daehyun-dong, Seodaemun-gu, South Korea; Faculty of Pharmacy, University of Surabaya, Raya Kalirungkut, Surabaya 60293, Indonesia; Faculty of Science, Brawijaya University, Malang 65145, Indonesia; Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine, Daejeon 305-811, 461-24 Jeonmin-dong, Yuseong-gu, South Korea; Natural Product Research Institute, Seoul National University, Seoul 110-460, South Korea
