E.L.C. Verkaar, H. Vervaecke, C. Roden, L. Romero Mendoza, M.W. Barwegen, T. Susilawati, I.J. Nijman, J.A. Lenstra
The genetic integrity of crossfertile bovine- or cattle-like species may be endangered by species hybridization. Previously, amplified fragment length polymorphism, satellite fragment length polymorphism and microsatellite assays have been used to analyze the species composition of nuclear DNA in taurine cattle, zebu, banteng and bison populations, while mitochondrial DNA reveals the origin of the maternal lineages. Here, we describe species-specific markers of the paternally transmitted Y-chromosome for the direct detection of male-mediated introgression. Convenient PCR-restriction fragment length polymorphism and competitive PCR assays are shown to differentiate the Y-chromosomes of taurine cattle, American bison and European bison, and to detect the banteng origin of Indonesian Madura and Bali cattle bulls.
Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, Yalelaan 8, Netherlands; Centre for Research and Conservation, Royal Zoological Society of Antwerp, Antwerp 2018, Koningin Astridplein 26, Belgium; Labocor, SL Poligono Indust. La Mina Nave 38, Colmenar, Viejo, Madrid, Spain; Roy. Inst. of Ling. and Anthropology, 2300 RA Leiden, Reuvensplaats 2, Netherlands; Animal Reproduction Laboratory, Animal Husbandry Faculty, Brawijaya University, Malang, Indonesia
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