Hermalina Sinay, Estri Laras Arumingtyas, Nunung Harijati, Serafinah Indriyani
Plant has a certain mechanism in adaptation to drought environment. The mechanism of plant adaptation to drought can be tracked to the molecular level by the drought stress induction and expression of some genes. One of the genes that induced by drought is dehydration responsive element binding or dehydration binding protein (DREB/DBP). The aim of this research was to identify the sequence of DREB gene of six local corn cultivars in Kisar Island South West Maluku Regency. Six local corn cultivars were obtained from local farmer in Kisar Island, and one reference variety as drought tolerance varieties originated from Research Institute of Cereals were planted in three replicates with complete randomized block design. Leaf sample collection for DNA analysis was harvested at the 65 days after planting. DNA isolation was conducted with using CTAB method. After success running PCR for amplification of DREB gene, the PCR product was sent to professional company for the sequencing process. The sequence result was aligned using the Clustal W alignment from Bioedit and the homology was compared with the same gene that already been reported in the data base of genebank. When compare with the DREB sequence of accession in the gene bank, the DREB gene sequence of local corn cultivars and reference variety showed similarity except nucleotide no 9. The nucleotide was deleted. It meant that DREB gene belong to local and reference corns was mutated. © 2015, Sphinx Knowledge House. All rights reserved.
Brawijaya University, Malang, East Java, Indonesia; Biology Education Programe, Pattimura University, Ambon, Maluku, Indonesia; Biology Departement, Brawijaya University, Malang, East Java, Indonesia
