Dahril, Wawid Purwatiningsih, Aulanni’am, Bambang S. Noegroho, Dany Hilmanto
Proprotein convertase subtilysin/Kexin Type4 (PCSK4) is a protein convertase enzymes in spermatozoa acrosome membrane which has molecular weight 54kDa, it has an important role in the acrosome reaction through endoproteolytic process that converts the precursor proteins into bioactive protein as a necessary substance to penetrate on the zona pellucida. This study aimed to characterize Proprotein Convertase Subtilisin/Kexin Type4 (PCSK4) of human spermatozoa to get an immunocontraception candidate. The research method; Characterization PCSK4 to identify the presence of convertase enzymes in the membrane proteins of human spermatozoa by immunohistochemistry technique, SDS PAGE and Western Blotting. The results of immunohistochemistry test showed that the molecule of PCSK4 54kDa expressed on the membrane of spermatozoa acrosome, the results of SDS PAGE showed that protein band which has molecular weight of 54kDa and confirmed as PCSK4 enzyme expressed and have possibility to develope as Male Contraception used as an antigen candidate of immunocontraception based on antibody againt to PCSK4. © 2014-2015, Sphinx Knowledge House. All rights reserved.
Padjadjaran University, Indonesia; Veterinary Medicine School, Brawijaya University, Indonesia; Biochemical Laboratorium, Brawijaya University, Indonesia
