U. Hatta, O. Sjofjan, I. Subagiyo, B. Sundu
Two studies on the effect of fermentation on nutrients contents of fermented copra meal, cellulases activity, bird performance and carcass percentage were carried out. In the first study, copra meal was fermented by Trichoderma viride with 4 levels of inoculum; L-1: without inoculum, L-2: 17.7 CFU (Coliform units) /g/kg copra meal, L-3: 35.4 CFU/g/kg copra meal, L-4: 53.1 CFU/g /kg copra meal, and 4 different incubation times; T-4: 4 days, T-6: 6 days, T-8: 8 days and T-10: 10 days. Fermented copra meal was analysed for nutrients and extracted to produce crude enzyme. Cellulase activity was analyzed by the DNS (3,5-dinitrosalicylic acid) method. In the second study, an enzyme product derived from fermented copra meal was tested in an in-vivo study. A total of 200 broiler chickens were kept for 6 weeks and given 5 different types of feeds: CM0: without copra meal; CM5-E: 5% copra meal + 0.5% crude enzyme; CM10-E: 10% copra meal + 0.5% crude enzyme; CM15-E: 15% copra meal + 0.5% crude enzyme; CM20-E: 20% copra meal + 0.5% crude enzyme). Feed and water were available at all times. The parameters observed were weight gain, feed intake, feed conversion ratio and breast meat percentage. The first study adopted a factorial randomized design (4 levels of inoculum, 4 incubation times and three replications), while the second study applied a completely randomized design with 5 treatments and 5 replicates (cages). Inoculum and incubation times significantly improved nutritive value of fermented copra meal. Celulase activity of crude enzyme was 0.84 g glucose/l. Growth and feed conversion ratio of birds fed a diet with 15% ot enzyme-treated copra meal were the same as in birds fed the control diet without copra meal. Breast meat percentage of birds fed copra meal diets supplemented with crude enzyme was similar to that from birds fed the control diet.
Tadulako University, Palu 94117, Indonesia; Brawijaya University, Malang, Indonesia; Brawijaya University, Malang 65145, Indonesia
