Ketut Edy Sudiarta, Karyono Mintaroem, Aulanni’am Aulanni’am, Mulyohadi Ali
Cervical cancer is the most common and the second mayor cause of cancer-related mortality gynecologyc malignancies in developing countries. Cancer stem cell (CSC) theory suggested, cancer is a stem cell diseases and related to the recurrence and drug resistant. Tumor suppressor gene RARß2 expression is reduced or undetectable inmany malignancy including cervical cancerwhich involved in multiple process carcinogenesis such as apoptosis signaling. The purpose of this study was to prove the anti tumor effect of DLEE (Dandelion leaves ethanolic extract) to Cervical stem cells and induce apoptosic through up-regulating the expression both gene and RARß2 protein. We conducted a control study using primary culture human cervical cancer stem cell treated with DLEE in various doses (6.25, 12.5, 25, 50, and 100 μg/mL) for 24 h. RARß2 gene confirmed by PCR technique and Agarose gel electrophoresis. The protein RARß2 expression were measured and confirmed by immunocytochemistry technique. The result showed the Significant (p<0.001) effect of the DLEE to induced apoptosis as 12.25, 16.25, and 23.75% after administration DEE with doses of 25, 50, and 100 μg/mL, respectively, compared with control value of 4.00%. And also have significant(p<0.00) effect to induction expression of RARß2 gene as 89.136, 102.738, 124.459 after induced DEE with doses of 12.5, 25, and 50 μg/mL, respectively. The dose effective was 100 μg/mL that increased protein RARß2 expression as 14.00%. In Conclusion, DLEEproved to have antitumor effect againstto cervical cancer stem cells by inducing apoptosis through up-regulated tumor suppressor gene RARß2 expression and induction RARß2 protein expression in primary culture cervical cancer stem cells. © 2015, Sphinx Knowledge House. All right reserved.
Department of Obstetric and Gynecology, Dr RamelanNaval Hospital dr. Ramelan, University Surabaya, Indonesia; Brawijaya University, Indonesia; School of Veterinary Medicine Brawijaya University, Indonesia
