Sri Wahjuningsih, Nurul Isnaini, Nolasco da Costa
During the process of in vitro maturation, oocyte requires proteins, hormones and growth factors for the survival and development. Unfortunetely hormones such as FSH, LH and estrogen are very expensive and imported. So alternative materials to replace these hormones with satisfactory results are being Follicular Fluid The research objective were to analyze the supplementation of Goat Follicular Fluid in medium on cumulus cell expansion and metaphase-II of the oocyte nucleus. The best quality of immature oocytes were matured in vitro for 26 h with basic medium TCM 199 + 10% FCS supplemented with different concentrations of Goat Follicular Fluid: 0%, 5%, 7.5%, 10%, 12.5% [v/v]. Observation of completely expanded of cumulus cells and the level of metaphase II were made on 26 h after in vitro maturation. The level of metaphase II was determined by staining 1% aceto-orcein and viewing under an inverted microscope with 400 X magnification. The results showed that supplementation of GFF 0%, 5%, 7.5%, 10%,and 12.5% yielded completely expanded of cumulus cells of 13.51%, 63.23%, 70.24%, 85.23%, and 65.42%, respectively while the oocyte reached to Metaphase-II was 12.45%, 52%, 66.42%, 75.20%, 67.27% for the respective GFF levels. It was concluded that supplementation 10% Goat Follicular Fluid yielded the highest rate of matured oocyte in vitro. © 2014 AENSI PUBLISHER All rights reserved.
Department of Animal Production, University of Brawijaya, Indonesia
