Ici Piter Kulu, Abdul Latief Abadi, Aminudin Afandhi, Nooraidawati
This aim of this research was to indentify B. bassiana by morphological and molecular identification that isolated from monoculture and polyculture field in central Kalimantan, Indonesia. Isolate of B. bassiana was explored from monoculture and polyculture field in Palangkaraya peatland, Central Kalimantan. Soil samples was taken from 60 sampling point nearby mustard plant root, 30 samples from monoculture field and 30 samples from polyculture field. Isolate of B. bassiana was identified by morphological and molecular characterization. The result showed that eight isolates of B. bassiana were found in Palangka Raya, Central Kalimantan from both monoculture and polyculture. There are no significant differences between B. bassiana isolates which isolated from monoculture and polyculture based on morphological and molecular characterization according PCR techniques. All of B. bassiana isolates has three size of band 800bp, 550bp, 400bp. B. bassiana could be potential entomopathogen agent from peatland in Palangka Raya, Central Kalimantan, Indonesia. © 2015 Sphinx Knowledge House. All rights reserved.
Brawijaya University, Malang, Indonesia; Palangka Raya University, Indonesia; Brawijaya University, Indonesia; Lambung Mangkurat Univeristy, Indonesia
