Sugiono, Simon Bambang Widjanarko, Loekito Adi Soehono
Fucoidanwere recovered from Sargassum polycystum by single-step extraction with ultrasonic wave pretreatment (amplitude 80%, 15 minute). Extraction wereoptimized using central composite design ofrespon surface method. Ultrasonic wave pretreatment conditions were 80%amplitude, for 15 minute. Alga solution in 0.03 MHCl ratio 1:20 (b/v) at70-90°C for 3-5 hour were evaluated duringthis process to establish a condition to maximize the extraction. The yield (%), total carbohydrate (mg/g), fucose (mg/g) and sulfate contents (mg/g) were also determined for eachexperimental condition. The result showed thatall extraction factors had significant effects (p<0.05) onfucoidan yield, fucose, total carbohydrate and sulfate contents. The optimum conditions were 81°C for 4.04 hours. The validated experimental crude fucoidanyield were7.15±5.58%, 131.15±1.92 mg/g fucose, 396.8±65.58 mg/gtotal carbohydrate and 94.67±1.37 mg/g sulfate contents. Characterization of crude fucoidan by FTIR and HPLC showed that crude fucoidan (Sargassum polycystum) is composed by fucose, xylose, galactose, rhamnose, glucose, mannose and sulfate.
Food Science and Technology Department, Brawijaya University, Malang 65141, Indonesia
