Wahyu Widowati, Laura Wijaya, Indra Bachtiar, Rimonta F. Gunanegara, Sri Utami Sugeng, Yudha Aryadi Irawan, Sutiman B. Sumitro, M. Aris Widodo
Mesenchymal stem cells (MSCs) from Wharton's jelly have a higher proliferation rate and self-renewal capacity than adult tissue-derived MSCs. A low oxygen level or hypoxic condition is prevalent in the microenvironment of the stem cells in the early stages of development. Hypoxia can influence proliferation and differentiation of various stem/precursor cell populations. This research was conducted: to determine the proliferation rate and characteristics of human MSCs from Wharton's jelly in hypoxic and normoxic condition; to evaluate their character after MSCs are incubated in hypoxic and normoxic environment using surface markers including CD105, CD73, CD14, CD19, CD34, CD45, and HLA-II; and to evaluate the proliferation rate and number of MSCs at many passages using the trypan blue method. The hypoxic and normoxic microenvironment showed significant differences in the proliferation rate and population doubling time, but and there were no differences in surface markers. © 2014.
Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia; Stem Cell and Cancer Institute, Jakarta, Indonesia; Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia; Department of Biology, Faculty of Science, Brawijaya University, Malang, East Java, Indonesia; Pharmacology Laboratory, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia
