Imam Hidayat, Rully Roesli, Zafrullah Arifin, Aulanni’Am Aulanni’Am
Cytochrome C, Caspase-8 and Poly (ADP-ribose) polymerase (PARP) are essentials proteases of the extrinsic and intrinsicapoptotic pathways. Understanding the proteases expression profile and its relationship to time period of cerebral damage post traumatic brain injury (TBI) become an important step in managing TBI. The aimed of this research was to evaluate the response of family protease enzymes CytochomeC and PRP (intrinsic pathway) and Caspase8(extrinsic pathway) afterTBI on rats. Rats were devided into 4 groups: observation of TBI after1h, 3 h, 6 h and 12 h. This study analyzed the expression of Cytochrome C, Caspase-8 and PARP byImmunohistochemistry technique. The results showed Cytocthe increasing of Cytochrome C tobe25%, 60%, 80% and 80%for 1 h, 3 h, 6 h and 12h after TBI, respectively. Meanwhile the Caspase-8 increased up to25%, 40%, 50% and 65% and PARP increases up to 40%, 65%, 78% and 85% in 1 h, 3 h, 6 h and 12 h after TBI, respectively. This findingsshowed traumatic brain injury (TBI) resulted different responses in proteases as an apoptotic marker. The study also showed that expression of Cytochrome C and PARP (intrinsic pathway) higher compared by Caspase-8 expression (intrinsic pathway). © 2015, Sphinx Knowledge House. All rights reserved.
General Surgery University of Syiah Kuala, Banda Ace, Indonesia; Padjajaran University Bandung, West Java, Indonesia; Brawijaya University Malang, East Java, Indonesia
