Pratiwi Pudjiastuti, Rosmawaty, Sri Sumarsih, Heny Arwati, Ari S. Nugraha, Loeki E. Fitri
Parasitemia is the quantitative content of parasites in the red blood cells, its indication of level of an active parasitic infection in human. Among the methods have been used for counting number of Plasmodium are optical microscopy (OM) and flow cytometry (FCM).The thin blood smear is stained by Giemsa for OM and DNA fluorescent stained detection for FCM. Croomine, epi-croomine and tuberostemonine are alkaloids isolated from the roots of Stemona tuberosa. The parasitemia of their alkaloids were determined in vitro using their methods above at 0.01; 0.1; 1 and 10 ppm concentrations, respectively. The fluorescent for DNA stain is used propidium iodide. The statistical analysis showed that the parasitemia determination on the two methods were almost similar.
Department of Chemistry, Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia; Department of Chemistry, Faculty of Mathematics and Science, University of Pattimura, Ambon, Indonesia; Department of Parasitology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Jember, Jember, Indonesia; Department of Parasitology, Faculty of Medicine, Brawijaya University, Malang, Indonesia
