Emad Eldin Amar Dagdag, Sukoso
The purpose of this study is to identify thermophilic bacteria isolated from Lapindo mud water and solid sediment with morphological and biochemical characteristics based on biochemical tests (microbact identification kits) compared to 16S rDNA test in Kagoshima University laboratory. The method used is exploratory method. Research was conducted by isolating dominant bacteria from water samples and sediment solid of Lapindo mud as isolates A3 and S3, then the characterization of morphological and biochemical properties of bacteria using morphological test colonies and bacterial cells. Biochemical tests were compared with test results of 16D rDNA then by testing the physical and chemical environmental parameters in Lapindo Sidoarjo mud. Results show that isolates A3 and S3 are included in the group of thermophilic bacteria that grow in an environment with temperature over 45°C. Thermophilic bacteria are known to have a thermostable enzyme that can be used in fish processing industries such as the manufacture of chitin and chitosan. From A3 and S3 isolates identification refer to the bacterial identification guidelines (Bergey’s Manual of Determinative Bacteriology), the possibility of species Pseudomonas pseudomallei with accuracy percentage of 99.48%. From these results compared with the results of identification using 16S-rDNA test of previous study, it was found that A3 isolate contains Pseudomonas stutzeri species and S3 isolatecontains Bacillus niabensis species. © 2015, Sphinx Knowledge House. All rights reserved.
Graduate School, University of Brawijaya, Malang, Indonesia; Al Mirqab and Tripoli University, Libyan Arab Jamahiriya; University of Brawijaya, Malang, Indonesia
