Ahmad Talib, Eddy Suprayitno, Aulani’am, Hardoko
Fish bone flour of Madidihang (Thunnus albacares Bonnattre) produced fine-shaped powder with yellowish white until yellow colour and has high content of Ca (calcium) and P (phosphorus). This research aims to determine the dose therapy of Madidihang fish bone flour deproteinization, non deproteinization and CaCO3 towards high calcium and phosphorus in the blood and the bone of the ovariectomy rat. We used experimental methods with 27 female wistar rat (Rattus novergicus) with age 75 day in therapy use of Madidihang fish bone flour and CaCO3 with doses of 0, 400, 800 and 1600 mg/kg bw day. Analysis of Ca and P parameters in blood serum and bone of rats is using AAS (automatic absorbance spectrophotometer). The results of in vivo test show that the highest value of Ca and P in blood and bone of rat is in the treatment of CaCO3 for 8.273 mg/dL; followed by non-deproteinization 7.951 mg/dL and deproteinization 7.127 mg/dL. While the highest P found in deproteinization treatment of 5.407 mg/g dw and the lowest in CaCO3 treatment for 2.014 mg/g dw. Otherwise, the highest Ca in bones of dose 1600 mg/g dw is deproteinization of 8.29 mg/g dw, and the lowest is CaCO3 for 7.04 mg/g dw. As for the highest dose deproteinization P 1600 2,224 and low CaCO3 1,475 mg/g bk. © 2014, Sphinx Knowledge House. All rights reserved.
University of Brawijaya, Indonesia; Laboratory of Biochemistry, University of Brawijaya, Indonesia
