Sri Rahayu, Susiati, Gatot Ciptadi, Dicky Mohamad Dikman
Bone Morphogenetic Protein 15 (BMP-15) was a member of the transforming growth factor-β (TGF- β) superfamily known to regulated ovarian functions in mammals. The aim of this research was to determine the BMP-15 gene polymorphisms of PO cattle. Random blood samples were collected from 30 animals. DNA was extracted from blood by QIAamp DNA blood mini kit (Qiagen). A 400 bp gene fragment was amplified by PCR (Polymerase Chain Reaction) using BMP-15 gene cattle specific primers (foward primer: 5'-AGTTTGTACTGAGCCGGTCT -3' and reverse primer: 5'-CTGACACACGAA GCGGAGT -3'). Restriction fragment length polymorphisms (RFLPs) in the amplified fragments were studied using MspI restriction enzyme. In this population, AA and AB genotypes have been identified with the 53.67 and 43.33 % frequencies, respectively. A and B alleles frequencies were 0.78 and 0.22, respectively. AA genotype is the dominant genotype and the A allele is the dominant allele. It can be concluded that MspI locus of BMP-15 gene of PO cattle was polymorphic.
Molecular Biology Laboratory, Biology Department, Brawijaya University, Malang 65145, Jl. Veteran, Indonesia; Husbandry Faculty, Brawijaya University, Malang 65145, Jl. Veteran, Indonesia; Centre Research of Local cattle, Pasuruan, Indonesia
