X. Mudawamah, M.Z. Fadli, X. Aulanni'am
To full fill the demand of domestic market, breeders make selection and mating just based on variations in phenotype. However so far, no molecular genetic studies with relation to feather colours in local Indonesian Canary. The molecular genetic will inform the variations of genetics in genes relating with feather colours in birds, but there has been no attempt to local canary. One of the genes related to feather colour in locus C chicken (albino or non-pigmented) is tyrosinase. The benefits of this research were expected to contribute to the conservation and exploitation of natural resources in Indonesia, especially on genetic variation and candidate genes associated with the colour of canary feathers. Total DNA was extracted from blood canaries polymerase chain reaction amplified DNA product was sequenced. The results showed that the amplification of canary genomes with tyrosinase gene were 200 bp fragment length while the results of the sequence had a length between 578-158 bp fragments. The genetic variation of gene tyrosinase in local Indonesian canary was shown in genetic similarity of 87-100 % and haplotype diversity of 0,9951 with 20 types.
Faculty of Animal Husbandry, Islamic University of Malang, Malang, Indonesia; Faculty of Medicine, Islamic University of Malang, Malang, Indonesia; Faculty of Chemical, University of Brawijaya, Malang, Indonesia